Overproduction of E. coli xylose isomerase

descriptionPublicationkeyboard_double_arrow_right Article 01 Jan 1986 English Publisher:Springer Science and Business Media LLCJournal:Biotechnology Letters, volume 8, pages 1-6 (issn: 0141-5492, eissn: 1573-6776,

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Authors: S. M. Lastick; M. Y. Tucker; V. Mackedonski; K. Grohmann;

doi: 10.1007/bf01044391

Overproduction of E. coli xylose isomerase

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Abstract

A promoterles DNA fragment containing theE. coli xylose isomerase gene and its ribosome binding site was ligated into a plasmid downstream from the strong λ PL promoter. The plasmid was then used to transformE. coli strains containing a temperature-sensitive λ repressor (cI857). The transformants overproduced xylose isomerase when the repressor was thermally inactivated.

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Beijing Solar Energy Research Institute
China (People's Republic of)

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