Construction of a shuttle vector betweenEscherichia coli andZymomonas anaerobia

descriptionPublicationkeyboard_double_arrow_right Article 01 Mar 1987 English Publisher:Springer Science and Business Media LLCJournal:Biotechnology Letters, volume 9, pages 163-168 (issn: 0141-5492, eissn: 1573-6776,

Copyright policy )

Authors: Ki Hong Yoon; M. Y. Pack;

doi: 10.1007/bf01024557

Construction of a shuttle vector betweenEscherichia coli andZymomonas anaerobia

- Summary
- Metrics

Abstract

A 1.7-kb cryptic plasmid was isolated fromZymomonas anaerobia and used to construct a shuttle vector inserting useful parts of pUC9, pBR322, and pRK2501.Escherichia coli was employed to clone the new plasmid designated pSR12. The 7.7-kb plasmid pSR12 reisolated from the host cells could transform competent cells ofZ.anaerobia at 2×10−7 frequency. This shuttle vector contains two antibiotic resistance markers, Kanr and Tetr, as well as restriction sites such as EcoRI, PstI, and XhoI, suitable for DNA recombinations.

Related Organizations

Korean Association Of Science and Technology Studies
Korea (Republic of)
KAIST

Impact byBIP!

	selected citations These citations are derived from selected sources. This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).	9
	popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.	Average
	influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).	Top 10%
	impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.	Top 10%