Picrosirius staining plus polarization microscopy, a specific method for collagen detection in tissue sections
Copyright policy )Picrosirius staining plus polarization microscopy, a specific method for collagen detection in tissue sections
Sirius Red, a strong anionic dye, stains collagen by reacting, via its sulphonic acid groups, with basic groups present in the collagen molecule. The elongated dye molecules are attached to the collagen fibre in such a way that their long axes are parallel. This parallel relationship between dye and collagen results in an enhanced birefringency. Examination of tissue sections from 15 species of vertebrates suggests that staining with Sirius Red, when combined with enhancement of birefringency, may be considered specific for collagen. An improved and modified method of staining with Sirius Red is presented.
- UNIVERSIDADE DE SAO PAULO Brazil
- Universidade de São Paulo Brazil
Intestines, Cartilage, Staining and Labeling, Histocytochemistry, Vertebrates, Animals, Collagen, Coloring Agents
Intestines, Cartilage, Staining and Labeling, Histocytochemistry, Vertebrates, Animals, Collagen, Coloring Agents
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