Structure and function ofl-glutamate decarboxylase

descriptionPublicationkeyboard_double_arrow_right Article 01 Mar 1991 English Publisher:Springer Science and Business Media LLCJournal:Neurochemical Research, volume 16, pages 227-233 (issn: 0364-3190, eissn: 1573-6903,

Copyright policy )

Authors: J Y, Wu; W M, Huang; L, Reed-Fourquet; J, Bao; B, Nathan; E, Wu; W H, Tsai;

doi: 10.1007/bf00966085

pmid: 1780025

Structure and function ofl-glutamate decarboxylase

- Summary
- Subjects
- Metrics

Abstract

Membrane bound L-glutamate decarboxylase (GAD) has been solubilized and partially purified from hog brain. The solubilized GAD appears to exist in two forms, alpha and beta, differing in their size and electrophoretic mobility. The alpha form has similar mobility as that of the soluble GAD in 7.5% and 5-25% gradient polyacrylamide gel electrophoresis suggesting that they are similar in size and charge. In addition, gene encoding for mouse brain GAD has been cloned and characterized. Mouse brain GAD cDNA consists of two DNA fragments with 1.6 and 1.0 Kb. The 1.6 and 1.0 Kb fragments contain 1657 and 974 bP, respectively. The significance of multiple forms of GAD is also discussed.

Related Organizations

Academia Sinica
Taiwan
University of Kansas
United States
Baylor College of Medicine
United States

Keywords

Base Sequence, Glutamate Decarboxylase, Octoxynol, Swine, Recombinant Fusion Proteins, Cell Membrane, Molecular Sequence Data, Brain, DNA, Polyethylene Glycols, Kinetics, Mice, Solubility, Animals, Electrophoresis, Polyacrylamide Gel

Impact byBIP!

	selected citations These citations are derived from selected sources. This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).	10
	popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.	Average
	influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).	Top 10%
	impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.	Top 10%