Use of a recombinantDictyocaulus viviparus antigen in an enzyme-linked immunosorbent assay for immunodiagnosis of bovine dictyocaulosis

Name: Use of a recombinantDictyocaulus viviparus antigen in an enzyme-linked immunosorbent assay for immunodiagnosis of bovine dictyocaulosis
Creator: T, Schnieder
Keywords: 0301 basic medicine, 0303 health sciences, Antibodies, Helminth, Cattle Diseases, Enzyme-Linked Immunosorbent Assay, Sensitivity and Specificity, Recombinant Proteins, 3. Good health, Dictyocaulus, 03 medical and health sciences

T, Schnieder

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Zeitschrift für Para...arrow_drop_down

Zeitschrift für Parasitenkunde Parasitology Research

Article . 1992 . Peer-reviewed

License: Springer TDM

Data sources: Crossref

Parasitology Research

Article . 1992

Data sources: Europe PubMed Central

https://dx.doi.org/10.1007/bf0...

Article

Data sources: Microsoft Academic Graph

Use of a recombinantDictyocaulus viviparus antigen in an enzyme-linked immunosorbent assay for immunodiagnosis of bovine dictyocaulosis

descriptionPublicationkeyboard_double_arrow_right Article 01 Jan 1992 English Publisher:Springer Science and Business Media LLCJournal:Parasitology Research, volume 78, pages 298-302 (issn: 0044-3255, eissn: 1432-1955,

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Authors: T, Schnieder;

doi: 10.1007/bf00937087

pmid: 1409529

Use of a recombinantDictyocaulus viviparus antigen in an enzyme-linked immunosorbent assay for immunodiagnosis of bovine dictyocaulosis

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Abstract

A specific recombinant antigen was evaluated for its immunodiagnostic potential in an enzyme-linked immunosorbent assay (ELISA). The antigen was used as a glutathione S-transferase (GST) fusion protein (DvGST3-14) or as a pure recombinant parasite protein (Dv3-14). A total of 55 sera from cattle experimentally infected with Dictyocaulus viviparus, 24 sera from naturally infected cattle and 25 sera from helminth-free cattle were examined. ELISA results obtained for the sera from experimental infections showed a calculated specificity of greater than 99% for both antigen preparations and a sensitivity of 93% (DvGST3-14) and 91% (Dv3-14). For field sera from natural infections, the specificity was calculated to be 90% (DvGST3-14) and greater than 99% (Dv3-14) and the sensitivity, 85% (DvGST3-14) and 70% (Dv3-14).

Related Organizations

Biology Centre
Czech Republic
Czech Academy of Sciences
Czech Republic

Keywords

Antibodies, Helminth, Cattle Diseases, Enzyme-Linked Immunosorbent Assay, Sensitivity and Specificity, Recombinant Proteins, Dictyocaulus, Predictive Value of Tests, Antigens, Helminth, Dictyocaulus Infections, Animals, Regression Analysis, Cattle

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