
doi: 10.1007/bf00839304
This paper presents data on the specific binding of naloxone with isolated rat enterocytes. Naloxone was bound with the cells in medium 199 containing 1 mg/ml of BSA. The incubation mixture contained 5 x 100 mM /sup 3/H-naloxone and, if indicated, other substances also. Dose dependence of binding of naloxone with rat enterocytes is shown. The kinetics of specific binding of naloxone with enterocytes at different temperatures is also shown, as is the irreversibility of binding of /sup 3/H-naloxone with isolated rat enterocytes. It was found that different ligands of opioid receptors can inhibit binding of naloxone competitively.
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