Hydroxylation in isolated liver cells

descriptionPublicationkeyboard_double_arrow_right Article 01 Oct 1975 English Publisher:Springer Science and Business Media LLCJournal:Bulletin of Experimental Biology and Medicine, volume 80, pages 1,193-1,194 (issn: 0007-4888, eissn: 1573-8221,

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Authors: I P, Kanaeva; A I, Archakov;

doi: 10.1007/bf00833157

pmid: 6092

Hydroxylation in isolated liver cells

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Abstract

Ethylenediamine tetraacetate and mechanical treatment were applied to obtain the isolated hepatocytes. Oxidative phosphorylation was found to be preserved in the isolated cells. Hepatocytes were capable of hydroxylation of dimethylaniline (DMA), ethylmorphine and aminopyrine. VMAX for the hydroxylation of DMA calculated as per 1 nmol of the cytochrome P--450 was higher in the cells than in the microsomes. NADH formed during the oxidation of glutamate and malate can be used for hydroxylation.

Keywords

Morphine Derivatives, Aniline Compounds, Citric Acid Cycle, Mitochondria, Liver, In Vitro Techniques, Hydroxylation, NAD, Oxidative Phosphorylation, Mixed Function Oxygenases, Rats, Liver, Microsomes, Liver, Animals, Aminopyrine, Cells, Cultured, NADP

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