Isolation of high-molecular-weight DNA from plasmids FB1 and FB1 drd of serotyped strains of Escherichia coli (AP1 and AP2)

descriptionPublicationkeyboard_double_arrow_right Article 01 Dec 1976 Russian Federation English Publisher:Springer Science and Business Media LLCJournal:Bulletin of Experimental Biology and Medicine, volume 82, pages 1,864-1,867 (issn: 0007-4888, eissn: 1573-8221,

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Authors: KALYUZHNAYA A.P.; MATVIENKO N.I.; SHCHIPKOV V.P.; PEKHOV A.P.;

doi: 10.1007/bf00785721

Isolation of high-molecular-weight DNA from plasmids FB1 and FB1 drd of serotyped strains of Escherichia coli (AP1 and AP2)

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Abstract

A method of isolating high-molecular-weight DNA from plasmids FB1 and FB1 drd of conditionally pathogenic strains ofEscherichiacoli has been developed. By electrophoresis of the DNA fragments in agarose gel and by sedimentation in a neutral glycerol concentration gradient the molecular weights of the isolated plasmid DNA were found to be of the order of 30·106 and 40·106, assuming two different plasmids, or 70·106 if only one.

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Russian Federation

Related Organizations

Peoples' Friendship University of Russia
Russian Federation

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