EcoRI-sensitive mutation of T7 phage

descriptionPublicationkeyboard_double_arrow_right Article 01 Jan 1977 English Publisher:Springer Science and Business Media LLCJournal:Molecular and General Genetics MGG, volume 150, pages 221-223 (issn: 0026-8925, eissn: 1432-1874,

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Authors: Y, Tsujimoto; H, Ogawa;

doi: 10.1007/bf00695402

pmid: 840225

EcoRI-sensitive mutation of T7 phage

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Abstract

It is known that DNA from phage T7 is not cleaved by restriction endonuclease EcoRI. However, DNA from phage T7am28 (gene 5) mutant (Studier, 1969) was found to be cleaved by the endonuclease at one site. The site is located at 0.46 fractional length from the left end of the molecule. The mutation which makes T7 DNA sensitive to the endonuclease is separable from the amber nutation and located between am28 and am233 (gene 6).

Related Organizations

Osaka University
Japan

Keywords

Genes, Genetic Linkage, DNA, Viral, Mutation, Endonucleases, Coliphages

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