The composition of the total epoxy acids obtained by saponifying the fraction of epoxyacyldiacylglycerols of the seed oil of Zeravshan sea buckthorn has been studied with the aid of high-resolution mass spectrometry and MD spectra. The most complete structural information was obtained by analyzing the spectra of the TMS derivatives of the MEs of the di- and tetrahydroxy acids formed on the opening of the epoxide rings. On the basis of the results of the mass-spectrometric study, structures are proposed for 10 mono- and four diepoxy acids and one triepoxy acid.