Synthesis of uridine diphosphate [1-3H]glucose and uridine diphosphate [6-3H]glucose

descriptionPublicationkeyboard_double_arrow_right Article 01 Jan 1987 English Publisher:Springer Science and Business Media LLCJournal:Chemistry of Natural Compounds, volume 23, pages 105-108 (issn: 0009-3130, eissn: 1573-8388,

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Authors: E. V. Snetkova; G. P. Akulov; L. S. Gordeeva; Yu. L. Kaminskii;

doi: 10.1007/bf00602470

Synthesis of uridine diphosphate [1-3H]glucose and uridine diphosphate [6-3H]glucose

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Abstract

Information is given on the synthesis of tritium-labeled UDPG by the successive transformation of D-[1-3H]glucose or D-[6-3H]glucose by the action of the enzymes hexokinase (EC 2.7.1.1), phosphoglucomutase (EC 2.7.5.1), and UDPG-pyrophosphorylase (EC 2.7.7.9) under conditions ensuring the retention of the tritium label. Methods of obtaining tritium-labelled substrates and intermediate products of their enzymatic transformations in the synthesis of [3H]UDPG are discussed.

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