The assay of ACTH and the action of ACTH and steroids on corticosteroidogenesis in the mouse
Copyright policy )The assay of ACTH and the action of ACTH and steroids on corticosteroidogenesis in the mouse
The optimum conditions are described for the assay of ACTH by its steroidogenic action on mouse adrenal glands. The index of precision averaged 0.10 and the assay can detect 1 imu ACTH/ml. High concentrations of β-oestradiol, testosterone, and corticosterone inhibited adrenal steroidogenesis acting at a site prior to the formation of pregnenolone. ACTH appeared to stimulate corticosteroidogenesis between cholesterol and pregnenolone. High concentrations of 3′5′ AMP also stimulated the glands. The specificity of the assay was good. Plasma slightly stimulated steroidogenesis other substances investigated had no effect at physiological concentrations.
- University of Leicester United Kingdom
Estradiol, In Vitro Techniques, Mice, Cholesterol, Glucose, Adrenocorticotropic Hormone, Adrenal Cortex Hormones, Pregnenolone, Adrenal Glands, Cyclic AMP, Animals, Female, Corticosterone, Progesterone
Estradiol, In Vitro Techniques, Mice, Cholesterol, Glucose, Adrenocorticotropic Hormone, Adrenal Cortex Hormones, Pregnenolone, Adrenal Glands, Cyclic AMP, Animals, Female, Corticosterone, Progesterone
selected citations These citations are derived from selected sources.This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).9 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Average influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 10% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Average
Citations provided by BIP!Popularity provided by BIP!