Preparation of homogeneous protamines by the fractionation of nucleoprotamine

descriptionPublicationkeyboard_double_arrow_right Article 01 Jan 1985 English Publisher:Springer Science and Business Media LLCJournal:Chemistry of Natural Compounds, volume 21, pages 353-355 (issn: 0009-3130, eissn: 1573-8388,

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Authors: N. V. Makarov; N. N. Yaglitskaya; V. K. Rybin;

doi: 10.1007/bf00574211

Preparation of homogeneous protamines by the fractionation of nucleoprotamine

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Abstract

A new method has been developed for obtaining individual protamines which is based on the chromatographic separation of an ultrasonically treated nucleoprotamine solution. The separation of the nucleoprotamine from the gonads of the Caspian sturgeonAcipenser stellatus on CM-Sephadex G-25 led to the isolation of three fractions. Analysis showed that two fractions contained homogeneous protamine — stellins A and B. The third fraction contained nonprotamines and DNA.

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