Freeze-fracture-autoradiography

descriptionPublicationkeyboard_double_arrow_right Article 01 Jan 1976 English Publisher:Springer Science and Business Media LLCJournal:Histochemistry, volume 50, pages 91-101 (issn: 0301-5564, eissn: 1432-119X,

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Authors: E, Rix; A, Schiller; R, Taugner;

doi: 10.1007/bf00495820

pmid: 1087634

Freeze-fracture-autoradiography

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Abstract

A new method for the electron microscope autoradiography of soluble substances in frozen tissue is described. The basic features of the method are freeze fracturing, the application of a suitable monolayer followed by exposure at low temperature and finally the separation of tissue and the replica-monolayer-sandwich after photographic processing. The advantages and limitations of the new method are discussed in terms of monolayer quality, contact, histochemography, resolution, freezing and recrystallisation artefacts.

Related Organizations

Heidelberg University
Germany

Keywords

Male, Ice, Animals, Autoradiography, Freeze Fracturing, Rana esculenta, Female, Diagnostic Errors

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