DNA-fluorometry of mammalian sperm

descriptionPublicationkeyboard_double_arrow_right Article 01 Jan 1977 English Publisher:Springer Science and Business Media LLCJournal:Histochemistry, volume 54, pages 1-7 (issn: 0301-5564, eissn: 1432-119X,

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Authors: J, Zante; J, Schumann; W, Göhde; U, Hacker;

doi: 10.1007/bf00493324

pmid: 914658

DNA-fluorometry of mammalian sperm

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Abstract

The Dna-content of sperm and testicular cells was measured by pulse-cytophotometry with high resolution. From flat sperm symmetric and narrow fluorescene distributions were obtained. Enzymatic treatment with papain or pronase and staining with an ethidiumbromide-mithramycin dye solution generate stoichiometric DNA-staining including that of mature sperm with a coefficient of variation below 2%.

Related Organizations

University of Münster
Germany

Keywords

Male, Spectrometry, Fluorescence, Species Specificity, Testis, Animals, Humans, DNA, Spermatozoa

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