Redundant DNA of Neurospora crassa
Copyright policy )Redundant DNA of Neurospora crassa
Approximately 20% of the DNA of Neurospora crassa consists of redundant sequences. This is calculated from the reassociation rate of fragmented, denatured DNA as measured by hydroxyapatite column chromatography. The redundant DNA has a complexity of 105 base pairs and a repetition frequency of up to 60 copies per genome. Its buoyant density in CsCl is 1.720 g/ml and its hypochromicity 20–24%. Base composition determination shows 54% GC content like Neurospora nuclear DNA. DNA-RNA hybridization studies indicate that rRNA and tRNA cistrons make up 2.3 and 1.2%, respectively, of the redundant fraction. Pulse-labeled RNA is shown to hybridize with both redundant and unique DNA fractions, suggesting that both fractions are transcribed.
- Johns Hopkins University United States
- JOHNS HOPKINS UNIVERSITY United States
Chromatography, Time Factors, Base Sequence, Neurospora crassa, Deoxyribonucleotides, Nucleic Acid Hybridization, DNA, Nucleic Acid Denaturation, Phosphates, Kinetics, Neurospora, RNA, Transfer, RNA, Ribosomal, Isotope Labeling, Centrifugation, Density Gradient, RNA, Spectrophotometry, Ultraviolet, Hydroxyapatites
Chromatography, Time Factors, Base Sequence, Neurospora crassa, Deoxyribonucleotides, Nucleic Acid Hybridization, DNA, Nucleic Acid Denaturation, Phosphates, Kinetics, Neurospora, RNA, Transfer, RNA, Ribosomal, Isotope Labeling, Centrifugation, Density Gradient, RNA, Spectrophotometry, Ultraviolet, Hydroxyapatites
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