Experiments were conducted in which sugarbeet plants (Beta vulgaris L. cv. Saxon) with 2 to 3 leaves were exposed to a simulated 2 day ozone episode (100 nl l−1, 7 h d−1). Three days later, the plants were sprayed with field rate phenmedipham (1.14 kg a.i. ha−1) and growth analysis conducted 7 days later indicated an antagonistic interaction was occurring. Physiological and biochemical studies were undertaken to determine the nature of this antagonism. Treatment with phenmedipham increased the ratio of transpiration to photosynthetic rates within 2 days of spraying, whilst exposure to ozone had no effect. When the two treatments were combined, water use efficiency was not significantly different from that when phenmedipham was applied alone. In contrast, trends in the membrane permeability after treatment, indicated that the response of plants exposed to ozone followed by treatment with the herbicide, was intermediate between that of the herbicide (high permeability) and ozone (low permeability). Furthermore, when the two treatments were combined the results of antioxidant enzyme assays indicated greater than expected activities of enzymes which are mainly cytosolic, eg. guaiacol peroxidase, as well as a similar increase in the activity of the mainly chloroplastic superoxide dismutase. Treatment with ozone alone and phenmedipham alone only slightly increased superoxide dismutase. Ozone may therefore induce the activities of these protective enzymes. Thus, when another oxidative stress, such as the photosystem II inhibitor phenmedipham, was applied the plants could then respond more quickly and showed less herbicide visible damage.