Enzymatic dehalogenation of 4-chlorobenzoate by 4-chlorobenzoate dehalogenase from Pseudomonas sp. CBS3 in organic solvents

descriptionPublicationkeyboard_double_arrow_right Article 01 Feb 1988 English Publisher:Springer Science and Business Media LLCJournal:Applied Microbiology and Biotechnology, volume 27, pages 577-580 (issn: 0175-7598, eissn: 1432-0614,

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Authors: Jürgen Thiele; Rudolf Müller; Franz Lingens;

doi: 10.1007/bf00451635

Enzymatic dehalogenation of 4-chlorobenzoate by 4-chlorobenzoate dehalogenase from Pseudomonas sp. CBS3 in organic solvents

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Abstract

4-Chlorobenzoate dehalogenase from Pseudomonas sp. CBS3 showed dehalogenating activity in various organic solvents. In alcohols like methanol (150%) or ethanol (120%) higher activities than in water (100%) were obtained. In apolar solvents like petroleum ether (5%) and nhexane (5%) only trace activities were observed. The solvents did not increase the stability of the enzyme. 4-Chlorobenzoic acid methylester, a substance not soluble in water, was not dehalogenated in organic solvents.

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University of Hohenheim
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