Selective spore survival during replica-plating of fission yeast

descriptionPublicationkeyboard_double_arrow_right Article 01 Jan 1977 English Publisher:Springer Science and Business Media LLCJournal:Archives of Microbiology, volume 112, pages 109-110 (issn: 0302-8933, eissn: 1432-072X,

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Authors: R, Egel;

doi: 10.1007/bf00446662

pmid: 843164

Selective spore survival during replica-plating of fission yeast

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Abstract

Vegetative cells of Schizosaccharomyces pombe, upon exposure to acetone vapours, are inactivated at a faster rate than ascospores of this yeast. This observation has been used to develop a simple and fast method by which colonies can be replica-plated and only spores survive in the replicas. The colony patterns are exposed to acetone while still on the velvet used for replica-plating.

Related Organizations

University of Freiburg
Germany

Keywords

Acetone, Ascomycota, Schizosaccharomyces, Methods, Spores, Fungal

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