
doi: 10.1007/bf00446647
pmid: 843163
Microbodies appearing abundantly in n-alkane-grown cells of Candida tropicalis pK 233 were isolated by means of sucrose density gradient centrifugation. Electron microscopical observation showed that the microbodies isolated were intact. Localization of catalase and D-amino acid oxidase in the isolated microbodies was confirmed. Isocitrate lyase, melate synthase and NADP-linked isocitrate dehydrogenase were also located in the microbody, but malate dehydrogenase, citrate synthase, aconitase and NAD-linked isocitrate dehydrogenase were not. Neither cytochrome P-450 not NADPH-cytochrome c reductase, the components involved in the n-alkane hydroxylation system of the yeast, were detected in the microbody fraction.
Organoids, Microscopy, Electron, Alkanes, Centrifugation, Density Gradient, Glyoxylates, Catalase, Cell Fractionation, Microbodies, Candida
Organoids, Microscopy, Electron, Alkanes, Centrifugation, Density Gradient, Glyoxylates, Catalase, Cell Fractionation, Microbodies, Candida
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