Bacteria, yeasts and fungi suspended in a dextran solution were added to ampoules containing strips of filter paper which were dried without vacuum conditions. The ampoules were sealed and stored in the dark at room temperature. Viability counts were made of the original suspension immediately after drying and after storage periods of 3-48 months. Although bacterial cultures of many general did not show much resistance against dry conditions, bacteria of 13 other general had survived well or moderately after 4 years of storage. Most of the dried yeast cultures had survived after this period. Of the 16 fungal genera tested, species of 6 genera exhibited growth after 4 years. Results of this study were compared with those of two other preservation methods by which the same microorganisms were used.