
doi: 10.1007/bf00436917
pmid: 3657903
Results related to the isolation, cultivation, culture and maintenance of the opportunistic pathogen Malassezia pachydermatis are reported. A dextrose nutrient medium with 1.5% yeast extract turned out to be the most favourable medium for its development. It permitted identification in 24 hours and maintenance of isolates for three months without subculturing. Addition of Tween 80 (1%) significantly enhanced the isolation of this yeast from clinical materials.
Dogs, Malassezia, Animals, Dog Diseases, Ear Diseases, Culture Media
Dogs, Malassezia, Animals, Dog Diseases, Ear Diseases, Culture Media
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