Isolation and characterization of a Neurospora glucose-repressible gene
Copyright policy )Isolation and characterization of a Neurospora glucose-repressible gene
Using differential hybridization, the cDNA copy of a Neurospora gene coding for an abundant glucose-repressible mRNA (grg-1) has been isolated. The cDNA was used to clone the genomic copy, and both were sequenced. The cDNA is nearly full length and contains putative translational start and termination codons. Conceptual translation indicates that grg-1 mRNA could direct the synthesis of a 7,000 molecular weight polypeptide. The genomic clone, contained in an 1,888 bp PvuII fragment, encompasses the entire cDNA as well as 838 bp of 5' and 369 bp of 3' flanking sequence. Comparison of the cDNA and genomic clones revealed the presence of two short introns in potential protein-coding sequences. grg-1 message levels were found to increase within minutes following the onset of glucose deprivation and rise 50 fold during the first 90 min of derepression.
- University at Buffalo, State University of New York United States
- State University of New York United States
- State University of New York at Potsdam United States
Base Sequence, Neurospora crassa, Transcription, Genetic, Genes, Fungal, Molecular Sequence Data, Restriction Mapping, Nucleic Acid Hybridization, RNA, Fungal, Blotting, Northern, Repressor Proteins, Blotting, Southern, Neurospora, Glucose, Gene Expression Regulation, RNA, Messenger, Cloning, Molecular, DNA Probes, DNA, Fungal, Transcription Factors
Base Sequence, Neurospora crassa, Transcription, Genetic, Genes, Fungal, Molecular Sequence Data, Restriction Mapping, Nucleic Acid Hybridization, RNA, Fungal, Blotting, Northern, Repressor Proteins, Blotting, Southern, Neurospora, Glucose, Gene Expression Regulation, RNA, Messenger, Cloning, Molecular, DNA Probes, DNA, Fungal, Transcription Factors
selected citations These citations are derived from selected sources.This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).104 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Top 10% influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 10% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Average
Citations provided by BIP!Popularity provided by BIP!