Determination of guanosine tetraphosphate (ppGpp) and adenosine pentaphosphate (pppApp) in various microorganisms by radioimmunoassay

descriptionPublicationkeyboard_double_arrow_right Article 01 Oct 1981 English Publisher:Springer Science and Business Media LLCJournal:Archives of Microbiology, volume 130, pages 134-137 (issn: 0302-8933, eissn: 1432-072X,

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Authors: Yasutaro Hamagishi; Akihiro Yoshimoto; Toshikazu Oki;

doi: 10.1007/bf00411065

Determination of guanosine tetraphosphate (ppGpp) and adenosine pentaphosphate (pppApp) in various microorganisms by radioimmunoassay

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Abstract

The intracellular levels of ppGpp and pppApp in various microorganisms were determined by radioimmunoassay, with was of more accuracy and convenience than previous 32P-labeling method. ppGpp was detected in bacteria, actinomycetes, yeasts and fungi. pppApp was found in non-spore forming bacteria such as the genera Pseudomonas and Escherichia, fungi and actinomycetes, but not in yeasts. In conclusion, ppGpp and pppApp are present in various prokaryotes and lower eukaryotes.

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Isuzu Advanced Engineering Center (Japan)
Japan

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