Electron microscopy of phages for Agrobacterium tumefaciens
Copyright policy )Electron microscopy of phages for Agrobacterium tumefaciens
Bacteriophages for three strains of A. tumefaciens were concentrated by ultracentrifugation, stained with 1% phosphotungstic acid (PTA), or 0.5% uranyl acetate, and examined with the electron microscope. Phage PT11 was a bacillary-shaped particle with a whip-like tail containing a knob at its distal end. Phage PIIBNV6 appeared to have an icosahedral head. The wide non-contractile tail terminated in a plate with pegs. Phage PIIBNV6-C was an icosahedral particle with a short, spike-like tail. Host cells of A. tumefaciens were encapsulated rods bearing polar or lateral flagella.
- University of Wisconsin–Oshkosh United States
- University of Wisconsin–Madison United States
Staining and Labeling, Phosphotungstic Acid, Acetates, Microscopy, Electron, Viral Proteins, Flagella, Uranium, Bacteriophages, Ultracentrifugation, Rhizobium
Staining and Labeling, Phosphotungstic Acid, Acetates, Microscopy, Electron, Viral Proteins, Flagella, Uranium, Bacteriophages, Ultracentrifugation, Rhizobium
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