Isolation of cobamides from Methanothrix soehngenii: 5-methylbenzimidazole as the ?-ligand of the predominant cobamide
Copyright policy )Isolation of cobamides from Methanothrix soehngenii: 5-methylbenzimidazole as the ?-ligand of the predominant cobamide
Methanothrix soehngenii was found to contain five different cobamides when grown on vitamin B12 supplemented as well as vitamin B12 free media. In both cases, it was shown by HPLC-chromatography and UV/VIS spectroscopy, that α-5-methylbenzimidazolyl-β-cyanocobamide was the predominant cobamide, accounting for 27% and 23%, respectively, of the total corrinoid content. Vitamin B12 and α-5-hydroxybenzimidazolyl-β-cyanocobamide could also be detected in both cell batches in varying amounts. Cells grown on vitamin B12 free medium contained significantly more baseless cobamides, indicating biosynthesis of cobamides.
- University of Zurich Switzerland
- Swiss Federal Institute of Aquatic Science and Technology Switzerland
acetotroph, 5-methylbenzimidazole, corrinoid, α-5-methylbenzimidazolyl-β-cyanocobamide, Methanothrix soehngenii, methanogen, vitamin B12, cobalamin, cobamide
acetotroph, 5-methylbenzimidazole, corrinoid, α-5-methylbenzimidazolyl-β-cyanocobamide, Methanothrix soehngenii, methanogen, vitamin B12, cobalamin, cobamide
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