Isolation and characterization of Hfr males in Citrobacter freundii
Copyright policy )Isolation and characterization of Hfr males in Citrobacter freundii
Citrobacter freundii Hfr donor strains were isolated from a C. freundii strain harbouring a temperature-sensitive factor F ts 114 lac+, by selecting for integrative suppression of the ts 114 mutation. Three Hfr strains were characterized, which transfer their chromosomes in a linear and oriented order. The first strain transfers: O-aro+-ilv+-pur+-thr+-leu+-pro+, the second: O-ilv+-pur+-thr+-leu+-pro+ and the third: O-ilv+-aro+-nad+-his+-pro+. The whole chromosome is transferred into the recipient cell within about 145 minutes. From these results we concluded that the linkage map of C. freundii is circular.
- University of Amsterdam Netherlands
Escherichia, Recombination, Genetic, Genetic Linkage, Conjugation, Genetic, Mutation, Escherichia coli, Chromosome Mapping, Lactose, Crosses, Genetic, Mutagens
Escherichia, Recombination, Genetic, Genetic Linkage, Conjugation, Genetic, Mutation, Escherichia coli, Chromosome Mapping, Lactose, Crosses, Genetic, Mutagens
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