The enzymic preparation of 14C-kaurene

descriptionPublicationkeyboard_double_arrow_right Article 01 Jan 1969 English Publisher:Springer Science and Business Media LLCJournal:Planta, volume 85, pages 171-174 (issn: 0032-0935, eissn: 1432-2048,

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Authors: J E, Graebe;

doi: 10.1007/bf00388547

pmid: 24515589

The enzymic preparation of 14C-kaurene

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Abstract

Endosperm from immature seeds of Cucurbita pepo L. converts 2-(14)C-DL-mevalonate to (14)C-(-)-kaurene with a yield of nearly 40% of the active isomer. Kaurene is the main product and the only diterpene hydrocarbon which is formed from mevalonate in the system and is therefore easily obtained radiochemically pure. The product was identified by thin-layer chromatography and recrystallization with authentic (-)-kaurene to constant specific radioactivity.

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