A radioimmunoassay for abscisic acid

descriptionPublicationkeyboard_double_arrow_right Article 01 Jan 1979 English Publisher:Springer Science and Business Media LLCJournal:Planta, volume 146, pages 139-145 (issn: 0032-0935, eissn: 1432-2048,

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Authors: D, Walton; W, Dashek; E, Galson;

doi: 10.1007/bf00388224

pmid: 24318051

A radioimmunoassay for abscisic acid

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Abstract

We have developed a radioimmunoassay (RIA) for abscisic acid (ABA) in the 0.1 ng to 2.5 ng range. Antibodies were obtained from rabbits immunized with ABA bound via its carboxyl group to bovine serum albumin. Cross-reactivity studies indicate that ABA esters are completely cross-reactive with ABA, while trans, trans abscisic acid (t-ABA) phaseic acid (PA) and dihydrophaseic acid (DPA) have much lower but significant cross-reactivities. Purification methods which reduce the levels of cross-reacting substances are described.

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State University of New York at Potsdam
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