Partial purification and properties of pectin lyase from Penicillium expansum

descriptionPublicationkeyboard_double_arrow_right Article 01 Sep 1993 English Publisher:Springer Science and Business Media LLCJournal:World Journal of Microbiology & Biotechnology, volume 9, pages 574-578 (issn: 0959-3993, eissn: 1573-0972,

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Authors: D O, Silva; M M, Attwood; D W, Tempest;

doi: 10.1007/bf00386297

pmid: 24420203

Partial purification and properties of pectin lyase from Penicillium expansum

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Abstract

A pectin lyase, poly(methoxygalacturonide) lyase, EC 4.2.2.10, from a culture filtrate of Penicillium expansum was partially purified 33-fold with 7.3% yield. The enzyme was monomeric with a molecular mass of 36.5 kDa. The enzyme did not contain pectate lyase activity and degraded citrus and apple pectin best at pH 7.0 and 40 to 45°C. The K m for citrus pectin was 9 mg ml(-1).

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University of Sheffield
United Kingdom

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