Plant tRNA nucleotidyltransferase

descriptionPublicationkeyboard_double_arrow_right Article 01 Jan 1978 English Publisher:Springer Science and Business Media LLCJournal:Planta, volume 142, pages 23-27 (issn: 0032-0935, eissn: 1432-2048,

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Authors: H, Cudny; M, Pietrzak; J, Kaczkowski;

doi: 10.1007/bf00385115

pmid: 24407993

Plant tRNA nucleotidyltransferase

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Abstract

A method for purification of tRNA nucleotidyltransferase from Lupinus luteus L. seeds on a preparative scale is presented. This involved ammonium sulfate fractionation and chromatography on DEAE-cellulose, hydroxylapatite, and QAE-Sephadex A-50. The final enzyme prepartion was apparently homogeneous and a 2000-fold purification was achieved. Data presented suggest the existence of only one form of tRNA nucleotidyltransferase in dry lupin seeds.

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