Rapid and simple assay for feruloyl and p-coumaroyl esterases

descriptionPublicationkeyboard_double_arrow_right Article 01 May 1996 English Publisher:Springer Science and Business Media LLCJournal:World Journal of Microbiology & Biotechnology, volume 12, pages 239-242 (issn: 0959-3993, eissn: 1573-0972,

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Authors: F H, O'Neill; L P, Christov; P J, Botes; B A, Prior;

doi: 10.1007/bf00360920

pmid: 24415230

Rapid and simple assay for feruloyl and p-coumaroyl esterases

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Abstract

A rapid, simple and sensitive method for detection of ferulic and p-coumaric acids using HPLC has been developed which can be used to determine the respective phenolic acid esterase activities of microorganisms. Prior concentration, purification or derivatization of the samples are not required. As little as 0.5 mg ferulic or p-coumaric acid/I could be detected and estimated in < 1 h. The method is specific for the two phenolic acids sice no interference by other components was observed.

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