The molecular weights of yeast ribosomal precursor RNAs
Copyright policy )The molecular weights of yeast ribosomal precursor RNAs
The molecular weights of the predominant rRNA precursors as well as those of 26-S and 17-S mature rRNA from Saccharomyces carlsbergensis were determined by polyacrylamide gel electrophoresis in the presence of formamide. Mature 26-S + 5.8-S rRNA was found to have a molecular weight of 1.24 X 10(6) while their immediate precursor, 29-S RNA, had a molecular weight of 1.52 X 10(6). Values of 0.70 X 10(6) and 0.82 X 10(6) were obtained for the molecular weights of mature 17-S rRNA and its 18-S precursor. Finally the 37-S precursor, common to both 29-S and 18-S RNA, was found to have a molecular weight of 2.80 X 10(6). Each precursor rRNA, therefore, contains extra sequences not found at the next stage of maturation.
- Vrije Universiteit Amsterdam Netherlands
Molecular Weight, Saccharomyces, RNA, Ribosomal, Electrophoresis, Polyacrylamide Gel, Nucleic Acid Denaturation
Molecular Weight, Saccharomyces, RNA, Ribosomal, Electrophoresis, Polyacrylamide Gel, Nucleic Acid Denaturation
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