Infection of spheroplasts of Pseudomonas with DNA of bacteriophage PM2

descriptionPublicationkeyboard_double_arrow_right Article 01 Sep 1971 Netherlands English Publisher:Springer Science and Business Media LLCJournal:Molecular and General Genetics MGG, volume 110, pages 263-271 (issn: 0026-8925, eissn: 1432-1874,

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Authors: Schans, G.P. van der; Weyermans, J.P.; Bleichrodt, J.F.;

doi: 10.1007/bf00337838

pmid: 5573498

Infection of spheroplasts of Pseudomonas with DNA of bacteriophage PM2

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Abstract

Spheroplasts of Pseudomonas BAL-31/PM2, obtained by treatment of the bacteria with lysozyme, can be infected with purified DNA from bacteriophage PM2. After 4 h of incubation the yield of progeny phage reaches a value of 107-6×107 plaque forming units/μg PM2 DNA. The yield increases linearly with the concentration of DNA over at least 3 orders of magnitude. The biological activity of double-stranded circular PM2 DNA containing one or more single-strand breaks per molecule (component II), does not differ significantly from that of intact PM2 DNA (component I). Single-stranded PM2 DNA obtained by denaturation of component II, and the irreversible alkali-denatured form of component I are also infective. © 1971 Springer-Verlag.

Country

Netherlands

Related Organizations

Delft University of Technology
Netherlands

Keywords

Bacteriophage typing, Genetics, Microbial, Ions, Lysozyme, Growth, development and aging, Metabolism, Pseudomonas, Virus DNA, DNA, Viral, Bacteriophages, Muramidase, Ion, Bacteriophage, Microbial genetics, Bacteriophage Typing

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