Restriction endonuclease mapping of pSC101 and pMB9

descriptionPublicationkeyboard_double_arrow_right Article 01 Jan 1978 English Publisher:Springer Science and Business Media LLCJournal:Molecular and General Genetics MGG, volume 164, pages 285-288 (issn: 0026-8925, eissn: 1432-1874,

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Authors: R C, Tait; H W, Boyer;

doi: 10.1007/bf00333158

pmid: 714015

Restriction endonuclease mapping of pSC101 and pMB9

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Abstract

A restriction endonuclease analysis of the plasmids pSC101 and pMB9 has allowed a determination of the alterations that occurred in the tetracycline resistance locus during the construction of pMB9 from pSC101. The genes for four of the polypeptides involved in tetracycline resistance have been positioned on the restriction endonuclease map of pSC101.

Related Organizations

University of California, San Francisco
United States

Keywords

DNA, Bacterial, Recombination, Genetic, Genes, Genetic Linkage, R Factors, Electrophoresis, Polyacrylamide Gel, DNA Restriction Enzymes, Tetracycline

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