Effect of DNA superhelicity on transcription termination
Copyright policy )Effect of DNA superhelicity on transcription termination
Restriction fragments containing either leut (a rho-independent transcription termination site) and/or leut' (a rho-dependent transcription termination site) were cloned into plasmid pOL4. Treatment of plasmid-containing Escherichia coli strains with coumermycin resulted in loss of in vivo plasmid superhelicity 10 min after antibiotic addition. Galactokinase levels specified by these plasmid-containing strains were the same regardless of whether functional DNA gyrase was present. These results suggest that transcription termination is unaffected by the superhelical state of DNA.
- Cornell University United States
Aminocoumarins, Chloramphenicol O-Acetyltransferase, Base Sequence, Transcription, Genetic, DNA, Superhelical, Anti-Bacterial Agents, Galactokinase, Acetyltransferases, Coumarins, Escherichia coli, Plasmids
Aminocoumarins, Chloramphenicol O-Acetyltransferase, Base Sequence, Transcription, Genetic, DNA, Superhelical, Anti-Bacterial Agents, Galactokinase, Acetyltransferases, Coumarins, Escherichia coli, Plasmids
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