A positive selection for mutants lacking orotidine-5′-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance

descriptionPublicationkeyboard_double_arrow_right Article 01 Nov 1984 English Publisher:Springer Science and Business Media LLCJournal:Molecular and General Genetics MGG, volume 197, pages 345-346 (issn: 0026-8925, eissn: 1432-1874,

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Authors: J D, Boeke; F, LaCroute; G R, Fink;

doi: 10.1007/bf00330984

pmid: 6394957

A positive selection for mutants lacking orotidine-5′-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance

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Abstract

Mutations at the URA3 locus of Saccharomyces cerevisiae can be obtained by a positive selection. Wild-type strains of yeast (or ura3 mutant strains containing a plasmid-borne URA3+ gene) are unable to grow on medium containing the pyrimidine analog 5-fluoro-orotic acid, whereas ura3- mutants grow normally. This selection, based on the loss of orotidine-5'-phosphate decarboxylase activity seems applicable to a variety of eucaryotic and procaryotic cells.

Related Organizations

Massachusetts Institute of Technology
United States
Whitehead Institute for Biomedical Research
United States

Keywords

Fungal Proteins, Microbiological Techniques, Orotic Acid, Carboxy-Lyases, Orotidine-5'-Phosphate Decarboxylase, Drug Resistance, Microbial, Saccharomyces cerevisiae

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