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Restriction enzyme analysis of the Plasmid ColIb DNA

descriptionPublicationkeyboard_double_arrow_right Article 01 Jan 1979Publisher:Springer Science and Business Media LLCJournal:Molecular and General Genetics MGG, volume 173, pages 197-201 (issn: 0026-8925, eissn: 1432-1874,

Authors: Anna Skorupska; Monika Buraczyńska; Z. Lorkiewicz;

doi: 10.1007/bf00330311

pmid: 386036

Restriction enzyme analysis of the Plasmid ColIb DNA

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Abstract

Plasmid ColIb (61.5 Mdal) was digested with restriction enzymes EcoRI and HindIII. The DNA digestion products were separated by electrophoresis on 1.2% agarose gels. There were identified 22 fragments of ColIb DNA generated by the endonuclease EcoRI and 21 fragments produced by HindIII. Molecular weights of the fragments were estimated. The total molecular weight of the fragments generated by EcoRI was 61.42 Mdal and for HindIII fragments 62.79 Mdal.

Related Organizations

Maria Curie-Skłodowska University
Poland

Keywords

DNA, Bacterial, Genes, Genetic Linkage, Escherichia coli, DNA Restriction Enzymes, Plasmids

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Fields of Science (3) View all

medical and health sciences

basic medicine

Fields of Science

medical and health sciences

basic medicine

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