Diffusion of Nutrients in a Biphasic Medium for the Cultivation of Trypanosomes

descriptionPublicationkeyboard_double_arrow_right Article 01 Sep 1973 English Publisher:Springer Science and Business Media LLCJournal:Zeitschrift für Parasitenkunde, volume 41, pages 231-238 (issn: 0044-3255,

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Authors: V, Stohlman; G, Mastright; B H, Kazan; S S, Kuwahara;

doi: 10.1007/bf00329489

pmid: 4721086

Diffusion of Nutrients in a Biphasic Medium for the Cultivation of Trypanosomes

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Abstract

1. Studies on uninoculated, biphasic media, containing 10% defibrinated rabbit blood in an agar base with Locke's solution as an overlay, have shown that diffusion of nutrients from the agar base into the overlay requires about 24–48 hr for equilibration.

Related Organizations

University of California, Irvine
United States
California State University, Long Beach
United States

Keywords

Trypanosoma, Time Factors, L-Lactate Dehydrogenase, Proteins, Alkaline Phosphatase, Culture Media, Enzymes, Agar, Blood, Cholesterol, Glucose, Albumins, Animals, Aspartate Aminotransferases, Rabbits, Amino Acids

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