Regulation of the lactose operon in vitro

descriptionPublicationkeyboard_double_arrow_right Article 01 Dec 1970 English Publisher:Springer Science and Business Media LLCJournal:Molecular and General Genetics MGG, volume 106, pages 296-306 (issn: 0026-8925, eissn: 1432-1874,

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Authors: Y, Iida; T, Kameyama; Y, Oshima; T, Horiuchi;

doi: 10.1007/bf00324047

pmid: 4921206

Regulation of the lactose operon in vitro

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Abstract

Using DNA of phage ϕ80p lac carrying lac-operon (i+o+z+y+), the transcription of the lac-operon was studied in vitro. The lac-specific mRNA having a molecular size of 28S was detected by hybridizing messenger RNA (mRNA) from induced cells (diploid for the lac-operon) to the ϕ80p lac DNA. The hybridization of lac-specific mRNA to ϕ80p lac DNA was almost completely competed by RNA (ϕ80p lac RNA) synthesized in the RNA polymerase reaction primed by ϕ80p lac DNA. Therefore it was concluded that ϕ80p lac RNA synthesized in vitro includes mRNA corresponding to an almost entire region of the lac-operon.

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Keywords

Time Factors, Lactose, RNA Nucleotidyltransferases, Tritium, Coliphages, Genes, DNA, Viral, Operon, Centrifugation, Density Gradient, Escherichia coli, Hybridization, Genetic, RNA, Messenger, Molecular Biology, Uridine

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