Isolation and characterization of a linear DNA plasmid from Streptomyces clavuligerus

descriptionPublicationkeyboard_double_arrow_right Article 01 Apr 1988 English Publisher:Springer Science and Business Media LLCJournal:Molecular and General Genetics MGG, volume 212, pages 172-176 (issn: 0026-8925, eissn: 1432-1874,

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Authors: C L, Keen; S, Mendelovitz; G, Cohen; Y, Aharonowitz; K L, Roy;

doi: 10.1007/bf00322461

pmid: 2836705

Isolation and characterization of a linear DNA plasmid from Streptomyces clavuligerus

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Abstract

A linear DNA plasmid (pSCL) has been isolated from Streptomyces clavuligerus by a method employing high concentrations of protease. Rate-zonal sedimentation on sucrose gradients was used to purify the plasmid. The plasmid is 12 kb in length and appears to be linked to protein at its 5' termini. A restriction endonuclease map of the plasmid for ten enzymes has been determined. Evidence for terminally repeated sequences is provided by cross-hybridization analysis.

Related Organizations

University of Alberta
Canada
Tel Aviv University
Israel

Keywords

DNA, Bacterial, Nucleotide Mapping, Nucleic Acid Hybridization, DNA Restriction Enzymes, Streptomyces, Plasmids

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