Structure of the Aspergillus niger pelA gene and its expression in Aspergillus niger and Aspergillus nidulans

descriptionPublicationkeyboard_double_arrow_right Article 01 Sep 1991 Netherlands English Publisher:Springer Science and Business Media LLCJournal:Current Genetics, volume 20, pages 293-299 (issn: 0172-8083, eissn: 1432-0983,

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Authors: Kusters-van Someren, M.A.; Harmsen, J.A.M.; Kester, H.C.M.; Visser, J.;

doi: 10.1007/bf00318518

pmid: 1934134

Structure of the Aspergillus niger pelA gene and its expression in Aspergillus niger and Aspergillus nidulans

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Abstract

Aspergillus niger pectin lyases are encoded by a multigene family. The complete nucleotide sequence of the pectin lyase PLA-encoding gene pelA has been determined. Comparison of the deduced amino acid sequence with the deduced amino acid sequence of the other characterized pectin lyase, PLD, shows that the proteins share 69% amino acid identity. When grown on media with pectin as the sole carbon source, A. niger transformants containing multiple copies of the pelA gene show raised mRNA levels and overexpression of the gene product PLA compared with the wild-type strain. PLA was purified and characterized. In A. nidulans transformants PLA is also produced in medium containing a high concentration of glucose and no pectin.

Country

Netherlands

Related Organizations

Wageningen University & Research
Netherlands

Keywords

Base Sequence, Blotting, Western, Genes, Fungal, Molecular Sequence Data, Gene Expression, Aspergillus nidulans, Chromatography, Affinity, Introns, Blotting, Southern, Pectin lyase, Transformation, Genetic, Protein Biosynthesis, Amino Acid Sequence, Aspergillus niger, Heterologous expression, Polysaccharide-Lyases

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