Gene replacement and ectopic integration in the zygomycete Mucor circinelloides

descriptionPublicationkeyboard_double_arrow_right Article 01 Sep 1993 English Publisher:Springer Science and Business Media LLCJournal:Current Genetics, volume 23, pages 542-546 (issn: 0172-8083, eissn: 1432-0983,

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Authors: J, Arnau; P, Strøman;

doi: 10.1007/bf00312649

pmid: 8319313

Gene replacement and ectopic integration in the zygomycete Mucor circinelloides

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Abstract

Transformation of Mucor circinelloides with plasmid pTL42 led to the identification of different integrative events. Analysis of eight transformed strains allowed the detection of three classes of transformants: integrative types derived from either homologous (exclusively at the leuA locus) or heterologous recombination and a class which originated by gene replacement. No autonomous replication of the vector was observed.

Related Organizations

Chr. Hansen (Denmark)
Denmark
Carlsberg Laboratory
Denmark

Keywords

Recombination, Genetic, Blotting, Southern, Transformation, Genetic, Mucor, Genetic Vectors, Restriction Mapping, Cloning, Molecular

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