A silver staining reaction for chromosomes and nuclei

descriptionPublicationkeyboard_double_arrow_right Article 01 Jan 1965 English Publisher:Springer Science and Business Media LLCJournal:Histochemie, volume 5, pages 78-85 (issn: 0948-6143, eissn: 1432-119X,

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Authors: C, De Martino; E, Capanna; M V, Civitelli; G, Procicchiani;

doi: 10.1007/bf00307894

pmid: 4161335

A silver staining reaction for chromosomes and nuclei

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Abstract

A staining reaction for the nucleus and chromosomes, using silver-hexamethylentetramine, buffered with borax, after hydrolisis with warm IN HC1, has been proposed. Its specificity has been assayed by means of enzymatic digestions (DNase, pepsin, trypsin), and through the comparison of the results with those obtained by the Feulgen reaction.

Keywords

Cell Nucleus, Silver, Staining and Labeling, Animals, Humans, In Vitro Techniques, Chromosomes

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