Dimethylsulfoxide as carrier in enzyme cytochemistry

descriptionPublicationkeyboard_double_arrow_right Article 01 Jan 1971 English Publisher:Springer Science and Business Media LLCJournal:Histochemie, volume 26, pages 93-94 (issn: 0948-6143, eissn: 1432-119X,

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Authors: J, Reiss;

doi: 10.1007/bf00307789

pmid: 4103372

Dimethylsulfoxide as carrier in enzyme cytochemistry

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Abstract

Addition of dimethylsulfoxide (DMSO) to the incubation medium of succinate dehydrogenase in a concentration of 10% enhances the staining reaction in the hyphae of the fungus Cercosporella herpotrichoides after an incubation period of 15 min. Controls without DMSO remain unstained. DMSO causes a rapid penetration of the components of the medium through the mucilage that covers the hyphae.

Related Organizations

Johannes Gutenberg University of Mainz
Germany

Keywords

Staining and Labeling, Histocytochemistry, Dimethyl Sulfoxide, Mitosporic Fungi

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