On the oxygen-sensitivity of various tetrazolium salts
Copyright policy )On the oxygen-sensitivity of various tetrazolium salts
1. Eight different tetrazolium salts have been chemically reduced with NADPH and PMS1 under oxygenated and oxygen-free conditions. 2. PMS has been shown to be able to remove all of the hydrogen from NADPH very rapidly, and to transfer all of this hydrogen onto tetrazolium salts, under suitable atmospheric conditions. 3. MTT, INT, TNBT, and NBT1 produced the same amount of formazan under both conditions; NT BT, TV, TT1 produced formazan under oxygen-free conditions, but produced no formazan under oxygenated conditions. 4. These results are explained on the basis of competition for the NADP Hhydrogen between oxygen and the four tetrazolium salts NT, BT, TV and TT.
Histocytochemistry, Tetrazolium Salts, In Vitro Techniques, Rats, Oxygen, Neoplasms, Animals, Indicators and Reagents, Oxidoreductases, Oxidation-Reduction, Hydrogen
Histocytochemistry, Tetrazolium Salts, In Vitro Techniques, Rats, Oxygen, Neoplasms, Animals, Indicators and Reagents, Oxidoreductases, Oxidation-Reduction, Hydrogen
selected citations These citations are derived from selected sources.This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).45 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Average influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 10% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 10%
Citations provided by BIP!Popularity provided by BIP!