The number of ribosomal RNA genes in Mycoplasma capricolum

descriptionPublicationkeyboard_double_arrow_right Article 01 Aug 1981 English Publisher:Springer Science and Business Media LLCJournal:Molecular and General Genetics MGG, volume 182, pages 502-504 (issn: 0026-8925, eissn: 1432-1874,

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Authors: M, Sawada; S, Osawa; H, Kobayashi; H, Hori; A, Muto;

doi: 10.1007/bf00293942

pmid: 6272066

The number of ribosomal RNA genes in Mycoplasma capricolum

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Abstract

We have examined the number of rRNA genes in Mycoplasma capricolum (KID) by hybridization of Bg/II-, EcoRI- and Xbal-digests of DNA to [3'-32P] 16S, 23S and 5S rRNAs according to the Southern procedure (1975). All the restriction gels gave two radioactive bands with three kinds of rRNA. Furthermore, band positions were indistinguishable from one another when 16S, 23S and 5S rRNAs were used as probes, indicating that each band contains sequences corresponding to the 3'-termini of 16S, 23S and 5S rRNAs. It is thus concluded that Mycoplasma capricolum chromosome carries at least two sets of genes for 16S, 23S and 5S rRNAs.

Related Organizations

Hiroshima University
Japan

Keywords

Mycoplasma, Genes, Genes, Bacterial, RNA, Ribosomal, Nucleic Acid Hybridization, DNA Restriction Enzymes

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