Immunofluorescent localization of calcitonin in the ultimobranchial gland of Rana temporaria and Rana pipiens

descriptionPublicationkeyboard_double_arrow_right Article 01 Jan 1971 English Publisher:Springer Science and Business Media LLCJournal:Histochemie, volume 26, pages 95-97 (issn: 0948-6143, eissn: 1432-119X,

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Authors: S, Van Noorden; A G, Pearse;

doi: 10.1007/bf00293499

pmid: 4934494

Immunofluorescent localization of calcitonin in the ultimobranchial gland of Rana temporaria and Rana pipiens

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Abstract

Calcitonin-like immunoreactivity has been demonstrated by immunofluorescence in the cells of the ultimobranchial gland of two species of Rana, using an indirect (sandwich) technique with anti-pure porcine calcitonin serum. The possibility of cross-reactivity, between the amphibian hormone and anti-porcine calcitonin antibody, had been anticipated on account of the observation that injected porcine calcitonin lowered the plasma calcium levels in one of the species under investigation.

Keywords

Antigen-Antibody Reactions, Calcitonin, Species Specificity, Immune Sera, Thyroid Gland, Animals, Fluorescent Antibody Technique, Anura

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