Gene sequence of recA + and construction of recA mutants of Vibrio cholerae

descriptionPublicationkeyboard_double_arrow_right Article 01 May 1994 English Publisher:Springer Science and Business Media LLCJournal:Molecular and General Genetics MGG, volume 244, pages 295-302 (issn: 0026-8925, eissn: 1432-1874,

Copyright policy )

Authors: U H, Stroeher; A J, Lech; P A, Manning;

doi: 10.1007/bf00285457

pmid: 8058040

Gene sequence of recA + and construction of recA mutants of Vibrio cholerae

- Summary
- Subjects
- Metrics

Abstract

The recA+ gene of Vibrio cholerae O1 has been cloned, its nucleotide sequence determined and the product characterized. A deletion mutation was constructed in the recA gene and mutants showed the typical sensitivity to UV and to DNA-damaging agents, as well as an inability to mediate homologous DNA recombination. The chromosomal recA deletion mutants in V. cholerae do not show altered virulence in the infant mouse cholera model and are thus ideal strains for use in complementation studies.

Related Organizations

University of Adelaide
Australia

Keywords

Base Sequence, Sequence Homology, Amino Acid, Virulence, Molecular Sequence Data, Restriction Mapping, Sequence Analysis, DNA, Biological Evolution, Mice, Rec A Recombinases, Genes, Bacterial, Mutation, Animals, Amino Acid Sequence, Cloning, Molecular, Vibrio cholerae

Impact byBIP!

	selected citations These citations are derived from selected sources. This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).	12
	popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.	Average
	influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).	Top 10%
	impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.	Average