Identification of trophoblast in chorionic villi biopsy samples

descriptionPublicationkeyboard_double_arrow_right Article 01 Feb 1986 English Publisher:Springer Science and Business Media LLCJournal:Human Genetics, volume 72 (issn: 0340-6717, eissn: 1432-1203,

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Authors: A E, Covone; P M, Johnson;

doi: 10.1007/bf00283940

pmid: 3510966

Identification of trophoblast in chorionic villi biopsy samples

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Abstract

We have investigated a test for rapid discrimination between foetal and maternal origin of chorionic villi biopsy samples. A monoclonal antibody named H315 reacting against a specific antigen present on the surface of foetal trophoblastic cells, plus a double-colour staining technique (FITC + PI), have been used for the identification of foetal cells (H315-positive) and for visualization of nucleate (PI-positive) and anucleate (PI-negative) structures of chorionic villi. This test could be useful in differentiating foetal and maternal cells in chorionic villi biopsy samples currently used for prenatal diagnostic purposes.

Related Organizations

Guy's Hospital
United Kingdom
University of Liverpool
United Kingdom
Guy's and St Thomas' NHS Foundation Trust
United Kingdom

Keywords

Antibodies, Monoclonal, Fluorescent Antibody Technique, Trophoblasts, Pregnancy Trimester, First, Pregnancy, Prenatal Diagnosis, Antigens, Surface, Humans, Female, Chorionic Villi

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